The 25-year-old Yates extended his advantage as Froome, 32, lost more time and is now nearly three minutes behind his compatriot.
The cyclists -- Holger Hagenbusch of Germany and Krzysztof Chmielewski of Poland -- had been traveling the world by bicycle.
The QMBL season 2017-2018 is a series of seven Cross Country Mountain Bike races which was started in September.
Froome hit the ground during his ride and suffered cuts and abrasions to the right side of his body, where his shorts and jersey were also torn.
Four-time Tour de France winner Froome is competing despite having returned an adverse analytical finding (AAF) during his victory at the Vuelta a Espana in September.
The former cycling superstar was due to face a trial next month over claims he defrauded the US government when he doped while racing for his US Postal Service-sponsored team.
Di Gregorio, of the Delko-Marseille Provence-KTM team, tested positive in a sample collected on March 8 for darbepoetin (dEPO), it said.
The 23-year-old Belgian was found unconscious and not breathing at the side of the road after racing along one of the course's famous cobbled sections
Goolaerts was found unconscious and not breathing at the side of the road on Sunday, after racing along one of the course's famous cobbled sections at Paris-Roubaix
Goolaerts died in hospital on Sunday at the age of 23, hours after suffering heart failure
Australia sealed a fourth track cycling gold when Stephanie Morton eased to victory in a one-sided women's sprint final
The 22-year-old Dane Mads Pedersen was nine seconds behind in second with last year's winner Philippe Gilbert of Belgium, also of Quick Step, third at 16sec.
Sky and Froome had hoped the UCI would find there were no grounds to penalise the four-time Tour de France winner
Armstrong had been invited by the organisers in one of the first signs of forgiveness within the cycling world over his doping past
Movistar's Valverde posted a total time of 28 hours, 25 minutes and 7 seconds in Barcelona to add to his 2009 and 2017 successes in the event at age 37.